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Intracytoplasmic Sperm Injection

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Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection (ISI)
It is an in vitro fertilization procedure in which a single sperm is injected directly into an egg. When the sperm count is very low, then a single sperm is injected in to the egg. Even if sperm are absent in ejaculate, and then sperm can be extracted from testis (Testicular Sperm extraction). It needs a micromanipulator with which an egg is held by suction and with a sharp needle a single sperm is injected and the fertilization is achieved. It is a boon for the couple where the routine IVF is not successful because of failed fertilization by conventional IVF or where the sperm count is very low or sperms are nonmotile, ICSI now offers men with poor semen parameters a chance of achieving a pregnancy.

Indications for ICSI are as follows
Men with an extremely low sperm count, low motility, or a high percentage of sperm with abnormal morphology. A low fertilization rate (or no fertilization) demonstrated by conventional IVF. Poor embryo quality felt to be secondary to a sperm problem with conventional IVF. Men requiring testicular aspiration or biopsy to obtain viable sperm. Long Distance and other Country patients can be admitted 21 st day of the previous menstrual cycle also. After IVF - ICSI, Results will be known in 2 weeks and successful patients can go back immediately.

Cryopreservation
Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures. At these low temperatures, any biological activity, including the biochemical reactions that would lead to cell death, is effectively stopped. However, when cryoprotectant solutions are not used, the cells being preserved are often damaged due to freezing during the approach to low temperatures or warming to room temperature. .

Embryo Donation
Embryo donation is a form of third party reproduction. In vitro fertilisation, or IVF, often results in a number of frozen, unused embryos after the woman for whom they were originally created has successfully carried one or more pregnancies to term. In embryo donation, these extra embryos are given to other couples or women for transfer with the goal of producing a successful pregnancy. The resulting child is considered the child of the woman who carries it and gives birth, and not the child of the donor, the same as occurs with egg donation or sperm donation. Typically, genetic parents donate the eggs to a fertility clinic or "embryo bank" where they are cryogenically preserved until a carrier is found for them. Typically the process of matching the embryo(s) with the prospective parents is conducted by the agency itself, at which time the clinic transfers ownership of the embryos to the prospective parents.

Embryo Freezing
Extra embryos are frozen in liquid nitrogen, if they are produced in greater number and of reasonable quality. These can be used in failed first cycle.

Sperm Freezing
It helps to store the sperms for long time and can be utilized whenever required. Like in absentia the insemination can be done. Before vasectomy the sperms can be stored. Before test tube baby sperms can be stored and on the day of insemination husband is not forced to produce the sample.

IVF Counseling
In Karpaga Viruksha Fertility and Research centre - couples entering the program are encouraged to attend a counseling session. For certain treatments however, such as those involving donor embryos or gametes, pre treatment counseling is compulsory. Further counseling sessions is available to all couples at all stages of treatment. Counselors are available and their services can be utilized for various reasons-if you have difficulty making a decision about treatment, have an unsuccessful treatment, are concerned about the impact of treatment on your relationship or simply want to talk over what you are experiencing. Counseling provides the opportunity to discuss your infertility treatment on a more personal level. It allows patients to raise issues that are more private, such as individual concerns, relationship difficulties, problems in adjusting to infertility, or current life situations that may affect the couple's experience of treatment.
One should also focus on the patients psychosocial and emotional needs by

  • Understanding childlessness,
  • Giving information and ensuring it is processed,
  • Implications counseling,
  • Support counseling and/or
  • Therapeutic counseling.

Surrogacy Programme
The term surrogacy is used when a woman carries a pregnancy and gives birth to a baby for another woman. Although the need for surrogacy is rare, there are still couples that require the need for a gestational carrier. A gestational carrier may be the only chance for a couple to have their own genetic child when the female partner is unable or capable of carrying a pregnancy on her own.